Bac protocol

Bac protocol

Download Bac protocol




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Date added: 20.03.2015
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Jun 28, 2007 - The protocol reported here is a simplified method used to produce and screen BAC libraries from Brachypodium species and other related

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NINDS GENSAT BAC TRANSGENIC PROJECT. Supported by NINDS Contract # N01-NS-7-2370. The Rockefeller University. 1. BAC Transgene Construction. Trimming BAC while simultaneously inserting ATI and ITK..17. Freezing a . You will follow the same protocol for transforming the BAC into SW105 cells or. BAC Cloning Protocol . Transformation of BAC-Optimized Replicator v2.0 Electrocompetent Cells . 11 Appendix C: Abbreviated Protocol .

This is a two step procedure, utilizing a Qiagen Large-Construct Kit for the initial purification, followed by either a chromatographic separation OR gel purification. 3. Frequently Asked Question – How to Sequence a BAC DNA Sample. 4. DNA Sequencing Protocol Question 3 – Preparing BAC DNA for Sequencing To ensure that the BAC DNA is pure enough to be used for downstream application, additional protocols have been elaborated to be combined with the protocolProtocol: Handling BACs. BACs (Bacterial Artificial Chromosomes) are synthetic vectors derived from E. coli F factor plasmids. These plasmids contain a very Keywords: homologous recombination guidelines BAC recombineering. Category: Protocol; Record History: Added on June 1, 2010 at 2:43 PM by Lindner, Jill CopyControl BAC Cloning Protocol. General Considerations. 1. Important! Users should avoid exposing DNA to UV light. Even exposure for short periods of time

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